The 155-pounders will headline the March 14 event, which will take place at American Airlines Center in Dallas, Texas.
Pettis will be riding a five-fight winning streak into the shootout. He returned from a yearlong layoff from a knee injury to make his first successful title defense last month against Gilbert Melendez.
Dos Anjos, meanwhile, solidified his next dibs status by dominating Nate Diaz in December. The Brazilian has won eight of his past nine scraps, with his lone blemish during his current run coming four fights ago to Khabib Nurmagomedov.
No other bouts have been reported for the lineup at this time.